X‐ray crystal structure of cytochrome P450 monooxygenase CYP101J2 from Sphingobium yanoikuyae strain B2

Structural elucidation of a hy­droxy–cineole product obtained from cytochrome P450 monooxygenase CYP101J2 catalysed transformation of 1,8-cineole

1,8-Cineole is an abundant natural product that has the potential to be transformed into other building blocks that could be suitable alternatives to petroleum-based chemicals. Mono-hydroxy-lation of 1,8-cineole can potentially occur at eight different carbon sites around the bicyclic ring system. Using cytochrome P450 monooxygenase CYP101J2 from Sphingobium yanoikuyae B2, the hy-droxy-lation c...

Crystal structure of cytochrome P450 MoxA from Nonomuraea recticatena (CYP105).

Cytochrome P450 MoxA (P450moxA) from a rare actinomycete Nonomuraea recticatena belongs to the CYP105 family and exhibits remarkably broad substrate specificity. Here, we demonstrate that P450moxA acts on several luciferin derivatives, which were originally identified as substrates of the human microsomal P450s. We also describe the crystal structure of P450moxA in substrate-free form. Structur...

Protein engineering of the cytochrome P450 monooxygenase from Bacillus megaterium.

Biosynthesis, Encodes a Putative Cytochrome P450 Monooxygenase

Phytoalexins are low molecular weight antimicrobial compounds that are synthesized in response to pathogen attack. The phytoalexin camalexin, an indole derivative, is produced by Arabidopsis in response to infection with the bacterial pathogen Pseudomonas syringae. The phytoalexin deficient 3 ( pad3 ) mutation, which causes a defect in camalexin production, has no effect on resistance to P. syr...

Cytochrome P450 Monooxygenase-Mediated Resistance to Insecticides

Insecticide resistance is one of the major obstacles to the control of agriculturally important arthropod pests. Resistance results in increased application frequencies, increased dosages, decreased yields, environmental damage, and outbreaks of human and animal diseases when vectors cannot be controlled. The World Health Organization has called insecticide resistance "the biggest single obstac...